Coding

Part:BBa_K3134005:Design

Designed by: Shiyuan Li   Group: iGEM19_Nanjing_high-school   (2019-10-16)


E.coli Cas1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 116
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 837
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For TfusCas3 can regulate the expression of Cas1Cas2, we use this protein to further develop our project.


Source

E.coli

References